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In our daily work,we often consider what kind of microscope is able to provide what we want to see.To solve the problem,we should figure out following questions first:

1.What is the dimension of things we want to see 2.We want detail or only prove its existence. 3.What is we want to see?Transparent or solid. 4.What is we want to see luminous body or nonluminous one.

Normally,there is a limit of resolution for optical microscope,when the limit is exceeded,the optical microscope will not be able to discern the observed object,this doesn’t mean can’t see any object smaller than the resolution ratio.Normally,the resolution ratio limit of the optical microscope is 200nm,in general situation,the bigger multiple the objective is,the higher resolution ratio it will reach and the bigger the aperture is,the higher resolution ratio it will be.The resolution ratio limit of our eye is 0.1mm,we can thereby reason that if the object we want to see clearly is 10um,the minimum multiple we need is 10×.When the eyepiece is 10×,we just need 1× objective to see whether the object exists.However,if we want to see the outline defects of this object,in this situation,the multiple we need will exceed 10× or higher.
 

Then in this situation,how can we choose microscopy camera according to our requirements?Microscopy camera as a kind of capturing recording device is to capture the image observed in the microscope and convert it to electronic format and save it in the computer for further analysis and research.Therefore,if we choose 10× and the detail we want to see is 0.5um,without considering of the objective limitation,we need at least 2000x2000 Pixel,that is about 4 Million pixels,and limited by the camera target surface dimension and color rendition requirement,this quantity of the pixels should reach 12 million to meet the minimum pixel quantity we demand.Actually,when we use 12 million pixel camera shooting,we can/t reach our expectation,it’s limited by the actual objective.Therefore,the megapixel camera should equiped with high numerical aperture objective to play its best.
According to the above algorithm,we can calculate the following result：

 

Objective	Numerical aperture	Theoretical resolution ratio 550nm	Vision field number mm	1/2 target surface 0.5multiple C mount camera visual field The minimum pixel for whole visual field	The required pixel number of the camera	The required pixel number of the camera	Considering the requirement of the human eye resolution,the recommending minimum camera pixel is :the pixel dimension of monitor is 0.27mm .	JPLY camera Model
10x	0.25	1.3um	22	16	9M Pixel	6.5M Pixel	8.6M Pixel	G1UD10C、 G1UC08C、G1UD14C
20x	0.4	0.84um	22	16	6M Pixel	4.4M Pixel	5.9M Pixel	G1UD05C、G1UC05C、G1UC08Cor higher pixel
40x	0.65	0.516um	22	16	3.6M Pixel	2.6M Pixel	3.5M Pixel	G1UD03C、G1UC05Cor higher pixel
100x	1.25	0.27um	22	16	1.9M Pixel	1.4M Pixel	1.9M Pixel	S1UC01C、G1UC01C

More information about the microscopic camera to buy,

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